Identification of the mutant with CotC-BmADH integration at <i>amyE</i> locus.

Abstract

<p>(A) Analysis of amylase activity. CotC-BmADH mutant strains and <i>B. subtilis</i> 168 (trp-) wide type grew on the starch-containing LB plate before (1) and after (2) being stained by iodine. The integration of CotC-BmADH might disrupt <i>amyE</i> and made the strain amylase deficient, while the while wide type strain showed a big white halo around colony due the secretion of amylase. (B) Site-directed PCR analysis using different primer pairs. Marker, <i>λ</i> DNA digested by <i>Eco</i>T14I; W: <i>B. subtilis</i> 168 (trp-) wide typ; M: CotC-BmADH mutant; primer pairs used in PCR are labeled below agarose gel.</p