Cloning strategy.
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Abstract
<p>(A) The construction of integration plasmid pJS700-BmADH. The fragments <i>amyE</i> 5′ and <i>amyE</i> 3′ in plasmid are homologous to the upstream and downstream of the amylase gene in <i>B. subtilis</i> 168 (trp-), respectively; <i>Em</i><sup>r</sup>, erythromycin resistant site; <i>CotC</i>, a <i>B. subtilis</i> spore coat protein encoding gene. (B) The schematic integration of CotC-BmADH to <i>amyE</i> locus. Arrows indicate the positions of primer pairs used in the site-directed PCR for confirmation of the correct integration.</p
