Experimental validation of results.

Abstract

<p>The figure shows the experimental validation results of the optimal drug combination for KSHV reactivation determined via the cross entropy algorithm. (A) Western blots showing KSHV lytic protein K8 expression 8 hr or 12 hr after drug treatment. The results were quantified as indicated in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0020998#s4" target="_blank">material and methods</a> section. (B) RT-Q-PCR showing the level of KSHV lytic transcripts ORF50 and PAN 4 hr or 8 hr after drug treatment. (C) Q-PCR of virion DNA copy-numbers measured 48 hours after treatment.</p