<i>MIP/AQP0</i> is activated by PITX3 via interaction with the proximal <i>bicoid</i> site, <i>bcd1</i>.

Abstract

<p><b>A.</b> Promoter activities of the <i>MIP656</i> reporters in human embryonic kidney cells <b>B.</b> Transactivation of <i>MIP656</i> reporters by PITX3 and its mutants in human embryonic kidney cells. Constructs and positions of <i>bicoid</i> sites are indicated on the left side. Wild-type <i>bcd1</i> or <i>bcd2</i> sites are depicted as open circles. Mutations (TAAT<u>CC</u> to TAAT<u>TT</u> substitutions) in <i>bcd1</i> or <i>bcd2</i> sites are depicted as dark circles with a strike-through. Student's paired t-Test with a one-tailed distribution was utilized to compare values. Experiments marked with asterisk (*) demonstrated a significant difference (P<0.001) in comparison to experiments performed with <i>MIP656</i> wild-type promoter (A) or <i>MIP656</i> wild-type promoter with PITX3-WT (B).</p