The ORP8 ligand-binding domain binds cholesterol.
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Abstract
<p><b>A</b> The purified His<sub>6</sub>-ORP8 ORD preparation, Coomassie blue stained SDS-PAGE gel. The two major bands (indicated, ORP8) represent the ORP8 ORD fusion protein. <b>B</b> The ability of His<sub>6</sub>-ORP8 ORD to extract [<sup>3</sup>H]cholesterol from unilamellar PC∶cholesterol (99∶1 mol%) vesicles was assayed. Purified GST was used as a negative control. Each assay contained 9.9 nmol PC and 100 pmol cholesterol; The protein amounts used (25–100 pmol) are indicated at the bottom. The data represents % of total DPM extracted from the vesicles, a mean ± s.e.m. (GST, n = 4; His<sub>6</sub>-ORP8 ORD, n = 5).</p
