Identification of the interacting domains in Nup62 and ORP8.

Abstract

<p><b>A</b> Schematic presentation of the Nup62 prey constructs used in yeast two-hybrid assays. The numbers indicate amino acid positions. <b>B</b> Interaction of the Nup62 constructs and ORP8m (lacking the C-terminal trans-membrane segment). Yeast colonies grown on SD/2- plates (left), SD/4- plates (middle) and x-gal assay (right) are shown. <b>C</b> Schematic presentation of the ORP8 bait constructs used. <b>D</b> Interaction of the ORP8 constructs with Nup62 in the yeast two-hybrid assay. Yeast colonies grown on SD/2- plates (top), SD/4- plates (middle) and x-gal assay (bottom) are shown. pGADT7 is the empty prey vector and pGBKT7 the empty bait vector.</p