Inability of <i>Grp94</i> KO HSCs to express surface integrin α4 and bind to fibronectin.

Abstract

<p>A) Representative flow cytometric analysis of CD49d and CD49e with BM LSKFlk2<sup>−</sup> and LSKFlk2<sup>+</sup> cells from WT and cKO mice. Grey-filled histogram represents isotype control staining; dashed green line represents WT cells; solid red line indicates cKO cells. B) The percentage of WT and cKO LSK cells bound to fibronectin <i>in vitro</i>. The number of cells binding to BSA was subtracted from that binding to fibronectin, the results then were normalized against the number of WT cells bound to BSA. The experiments were performed twice in duplicate; each replicate contained pooled BM from 2 to 4 WT or cKO mice. The data are presented as mean ± s.e..</p

    Similar works

    Full text

    thumbnail-image