Effect of LPS on TNF-αmRNA expression in RA FLS and THP-1 cells.

Abstract

<p><b>A, B.</b> TNF-α mRNA expression was determined by RT-PCR in RA FLS (<b>A</b>) and THP-1 cells (<b>B</b>) stimulated with LPS (1 µg/ml) for 2 h, 4 h and 6 h. Control cells were incubated for 4 h with medium (C). <b>C, D.</b> RA FLS and THP-1 cells were stimulated for 3 h with LPS and then incubated for another 1, 2, 3 and 4 h with actinomycin D (5 µg/ml). Control cells were incubated for 3 h with medium. TNF-α mRNA expression was determined by RT-PCR. The results are representative of three different experiments for each patient.</p