PDFR-MYC and CRYPTOCHROME are precisely co-expressed in the same subsets of clock neurons.

Abstract

<p>PDFR-MYC fly brains were triple-stained with anti-MYC (green), anti-PER (magenta), and anti-PDF (blue) antibodies (A, C, E), or double-stained with anti-MYC (green) and anti-CRY (blue) antibodies (B, D, F). (A) Three LNds of six show strong PDFR-MYC staining (white arrowheads), whereas the others show no PDFR-MYC (magenta arrowheads). (B) Three LNds express both PDFR-MYC and CRY (arrowheads). (C) The 5^th s-LNv showed strong staining of PDFR-MYC (arrow). (D) Nine LNv stained with anti-CRY antibody, three of these were also stained with anti-MYC. By reference to results shown in panel C, we assigned the strongest MYC expressing neuron to the 5^th s-LNv (arrow). The two remaining MYC(+) neurons are marked with white arrowheads: By size, we speculate these are l-LNv. Two CRY(+) l-LNvs (by size) were not detected with anti-MYC antibody (magenta arrowheads). (E) Six of the 17 DN1s show PDFR-MYC staining at strong levels (white arrowheads), whereas the remaining ones show little or no MYC staining. (F) Six of 15 DN1ps express both PDFR-MYC and CRY (arrowheads). Asterisks (in A, B, D, F) - non-specific staining by either anti-MYC or anti-CRY rabbit antibodies. Scale bars, 10 µm. (G) A summary diagram of the precise PDFR and CRY co-expression in discreet subsets of the three major pacemaker cell groups.</p