PER molecular rhythms in double mutants under different light intensity LL conditions.

Abstract

<p>(A–D) low intensity; (E–H) high intensity. (A, E) PDF(+) s-LNv; (B, F) 5^th s-LNv; (C, G) ITP(+) LNd; (D, H) DN1. Filled histograms - nuclear values and open histograms - cytoplasmic values (B, C, F, G). (A, E) PDF(+) s-LNv (magenta) showed robust PER (green) staining rhythms under both light intensity conditions. (B–D, F–H) For the other cells examined, a statistically-significant amplitude rhythm was shown under low light conditions for the 5^th s-LNv and for the ITP(+) LNd. None of the cells showed a significant amplitude rhythm under high light intensity. (B) At low intensity, both nuclear and cytoplasmic peaks in the 5^th s-LNv occurred at CT77: nuclear amplitude rhythm – 2.2-fold; cytoplasmic amplitude rhythm – 2.5-fold. ANOVA test revealed that the difference in this group is significant (P<0.0003). (C) In the ITP(+) LNd, both nuclear and cytoplasmic peaks occurred at CT77: nuclear amplitude rhythm – 3.3-fold; cytoplasmic amplitude rhythm – 2.0-fold. ANOVA test revealed the group difference is significant (P<0.0001). (D) PER(+) DN1 neurons were counted at four time points under low light intensity conditions. The rhythm in PER(+) DN1 didn't show 24 hour rhythms. ANOVA test P value was 0.0038. (F) At high intensity, nuclear peak in the 5^th s-LNv occurred at CT77 and cytoplasmic peak occurred at CT71: nuclear amplitude rhythm – 1.8-fold; cytoplasmic amplitude rhythm – 1.4-fold. ANOVA test indicated the group difference was not significant (P = 0.0527). (G) In the ITP(+) LNd, the nuclear peak occurred at CT83 and the cytoplasmic peak at CT77: nuclear amplitude rhythm – 2.3-fold; cytoplasmic amplitude rhythm – 1.4-fold. ANOVA test revealed that the difference in this group is not significant (P = 0.08). (H) PER(+) DN1 neurons were counted at four time points under high intensity conditions. The rhythm in PER(+) DN1 showed a peak at CT65, but it was not statistically significant. Results from post-hoc statistical tests are presented in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018974#pone-0018974-t002" target="_blank">Table 2</a>.</p