The PCA production was negatively regulated by GacA via <i>phzA2-G2</i> cluster mainly at post-transcriptional level.

Abstract

<p>PCA production and cell growth curves in single <i>gacA</i> inactivated mutant M18ΔG (square), double <i>gacA</i> and <i>phzA1-G1</i> mutant M18ΔGΔP1 (triangle) or double <i>gacA</i> and <i>phzA2-G2</i> mutant M18ΔGΔP2 (diamond) (A). The cell growth and expression of transcriptional fusion pMP2C (B) or translational fusion pMP2L (C) in wild type strain M18 (circle) and mutant M18ΔG (square). Symbols: solid, growth curves; empty, PCA production or β-Galactosidase activities. All experiments were performed in triplicate, and each value is presented as the average ± standard deviation.</p

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