CASZ1a and CASZ1b are coordinately regulated.
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Abstract
<p>A. Neuroblastoma cell lines were treated with 2 µM demethylating agent 5-Aza-dC for 3 days. Both CASZ1a and CASZ1b mRNA levels were upregulated in KCNR, NGP and SY5Y as detected by RT-PCR using CASZ1a and CASZ1b specific primer (all p<0.02). B. NGP cells were treated with the class I histone deacetylase inhibitor, depsipeptide (5 ng/ml) for 24 hr. Both CASZ1a and CASZ1b mRNA levels were up-regulated by depsipeptide as detected by RT-PCR (all p<0.0002). C. Both CASZ1a and CASZ1b mRNA levels were up-regulated by 5 µM RA in SY5Y cells as detected by RT-PCR (all p<0.05). D. Both mCASZ1a and mCASZ1b mRNA levels were up-regulated when mouse C2C12 cells are cultured in 2% horse serum for 4 days or 8 days as detected by RT-PCR (all p<0.001). E. CASZ1b mRNA levels was down-regulated and CASZ1a mRNA levels was up-regulated by 2 µM dibutyryl cyclic AMP treatment for 48 hr in KCNR cells as detected by RT-PCR (all p<0.05).</p