<p>A. Induction of CASZ1b by Tet in SY5YtetCASZ1b cells inhibited cell growth, and the magnitude of inhibition was similar to CASZ1a in SY5YtetCASZ1a cells. The cell confluence was measured at different time point using IncuCyte instrument (P<0.05 for all time points since day 2). B. Similar to CASZ1a, the induction of CASZ1b by Tet inhibited proliferation of SY5YtetCASZ1b cells as assessed using a MTS assay on day 7 (p<0.005). C. Similar to CASZ1a, the induction of CASZ1b by Tet decreased clonogenicity of SY5YtetCASZ1b cells in soft agar (p<0.005). D. SY5YtetCASZ1b cells were subcutaneously injected into nude mice. The induction of CASZ1b expression significantly inhibited tumor growth in the xenograft model (n = 5 mice per group, total 10 mice; Volume = long × short<sup>2</sup>/4; data are shown as mean ± SEM, all time point p<0.02 since day 45).</p
