EBA-175 is expressed in <i>P.falciparum</i> schizonts, and late liver stages in human hepatocytes <i>in vitro</i>, but not sporozoites.

Abstract

<p>A) Sporozoites stained with anti-PfCSP mAb 2A10 (used at 0.136 ug/mL), B) sporozoites stained with mAb R217 (used at 300 ug/mL), C) schizont stained with mAb R217 (used at 2.34 ug/mL), <b>M</b>: merozoite; <b>A</b>: apical end of the merozoite expressing EBA175, D) schizont stained with anti-PfCSP mAb 2A10 (used at 68 ug/mL). The nuclei were stained with DAPI, E) HC-O4 human hepatocytes stained with anti-<i>P.falciparum</i> liver stage antigen -1 (PfLSA-1) polyclonal rabbit serum (1:50 dilution) 6 days post infection with <i>P.falciparum</i> sporozoites, F) HC-O4 human hepatocytes stained with mAb R217 (used at 100 ug/mL) 6 days post infection with <i>P.falciparum</i> sporozoites. N: nucleus of the hepatocyte; P: liver stage parasite.</p