<p>(A) Schematic diagram of the <i>AtCYN</i> gene and the T-DNA insertion positions. Gray boxes represent exons. Lines above the gene indicate T-DNA insertion positions. Accession numbers of the <i>Atcyn</i> mutant lines was listed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0018300#pone-0018300-t004" target="_blank">Table 4</a>. (B) Northern blot analysis of <i>AtCYN</i> transcripts in <i>cyn</i> mutants. Total RNA was isolated from 14-day-old seedlings. Different homozygous individuals identified were analyzed in different lanes. Blot signals (indicated by the arrow) were quantified with ImageJ version 1.4 software and the values are presented below each lane. The 5S rRNA was visualized with ultraviolet light and was the loading control. (C) Quantitative RT-PCR analysis of <i>AtCYN</i> transcripts in Col 0, <i>cyn7</i> and transgenic plants. Error bars represent the standard deviation of three biological replicates. (D) Semi-quantitative analysis of HA:AtCYN and HA:OsCYN in transgenic plants using western blotting (WB). Blot signals (indicated by the arrow) were quantified with ImageJ and the values are presented below the lanes. The large subunit of Rubisco was visualised by Coomassie Brilliant Blue (CBB) and was the total protein loading control (indicated by the arrow).</p
