<p>Cells were incubated with or without 50 ng/ml IL-17A, IL-17F or IL-22 for 6 days, then fixed and immunolabeled with ZO-1 or occludin. Immunostaining for ZO-1 and occludin in untreated or IL-22-treated ARPE-19 monolayer showed a continuous labeling in the region of cell-cell contact. Incubation with IL-17A or IL-17F caused a marked disruption of ZO-1 and occludin staining. The immunostaining shown is representative of three independent experiments. Scale bar = 15 µm.</p
