Gel filtration and cyanase activities of His-tagged AtCYN, OsCYN and AtCYN mutants (E94L and S117A).

Abstract

<p>(A) Gel filtration. High Molecular Weight (HMW) Standard: Thyroglobulin, 669 kDa; Ferritin, 440 kDa; Aldolase, 158 kDa; Conalbumin, 75 kDa and Ovalbumin, 43 kDa. And calculated molecular weight: AtCYN, 210.74 kDa; OsCYN, 211.72 kDa; AtCYN-E94L, 71.64 kDa and AtCYN-S117A, 62.76 kDa. (B) Cyanase activities of His-tagged AtCYN, OsCYN and AtCYN mutants (E94L and S117A) at pH 7.7 and 27°C.</p