Histopathological analysis of lungs, spleens, and livers 6 weeks post-vaccination at 72 h post-challenge from mice vaccinated six weeks earlier.

Abstract

<p>Lungs of S₄₀ + E₁₀ vaccinated mice (v) were free of any histopathological lesions and bacteria and were similar to lung tissue from unimmunized and uninfected control mice (i). No bacteria, necrosis or edema were present in spleens of the S₄₀ + E₁₀ vaccinated mice (x) and histology was similar to healthy spleen tissue (vi). The livers of the S₄₀ + E₁₀ vaccinated mice (xv) did not show any lesions similar to control mice (xi). Av - Alveoli, F - Follicle, Mz - Marginal zone, and RP - Red pulp. Arrow - bacteria, black arrowhead - neutrophilic infiltration, yellow arrowhead - lymphocytic infiltrations, * – edema, and + - necrotic cells. Objective lens magnification is 100X. Scale bar  = 20 µm.</p