<p><b>A</b>, <i>RASSF1A</i> promoter activity by GFP expression under the control of different length of RASSF1A promoter segments. A predicted p53 binding site in <i>RASSF1A</i> promoter (−2718 bp) was showed in the construction c. <b>B</b>, CpG island prediction of <i>RASSF1A</i> by software MethPrimer online (<a href="http://www.urogene.org/methprimer/" target="_blank">http://www.urogene.org/methprimer/</a>). Predicted CpG island is indicated by blue color. <b>C</b>, Representative results of the methylation-sensitive PCR (MSP) analysis of <i>RASSF1A</i> in both seminoma and nonseminoma. M, MSP PCR; U, unmethylation-sensitive PCR. <b>D</b>, DNA methylation status of individual CpG sites by sodium bisulfite sequencing analysis. Black and white circles represent methylated and unmethylated CpGs respectively.</p
