<p><b>A</b>, <i>RASSF1A</i> promoter activity was inhibited by p53 protein with a dose dependent manner. pRASSF1A-GFP (construction c in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0017017#pone-0017017-g001" target="_blank">Fig. 1</a>) was co-transfected with different concentration of CMV-p53 into COS-7 cells, and transfected cells were analyzed by the flow cytometry. <b>B</b>, pRASSF1A-GFP was transfected into COS-7 cells (<b>a</b>) or co-transfected with p53 (<b>b</b>), and images were taken under fluorescent microscopy. p53 remarkably decreased expression of RASSF1A-GFP. GFP was mainly expressed in the cytoplasm. Nuclei were stained with Hoechst. <b>C</b>, Overexpression of p53 in Siha cells decreased RASSF1A protein levels, revealed by Western blot analysis of RASSF1A and p53 expression using anti-RASSF1A or p53 antibody after transfection of the p53 expression plasmid into Siha cells. Lysates of non-transfected cells and cells transfected with the pcDNA3 vector were used as controls. β-actin was used as an internal control. Molecular weights of the proteins are shown on the right.</p
