The effects of NPPB and DCPIB on OGD induced pyramidal neuron death.

Abstract

<p>Besides the control group (in aCSF, <b>A</b>), the hippocampal slices were randomly divided into three groups after 25 min OGD treatment to receive the following post-OGD treatment: 1) in bath solution (<b>B</b>. OGD); 2) bath solution+100 µM NPPB (<b>C.</b> NPPB); and 3) bath solution+10 µM DCPIB (<b>D.</b> DCPIB). The fluorescence density of the TO-PRO-3-I staining is proportional to the neuronal death. Between the aCSF control and the OGD group, the neuronal death increased by 5.3 fold (3.2±0.6 in aCSF <i>vs</i>. 16.9±1.9 in OGD, n = 13). The neuronal death was reduced to 6.0 ±0.5 (n = 20) by 100 µM NPPB, and to 9.4±1.1 (n = 20) by 10 µM DCPIB. Both NPPB and DCPIB were added in the reperfusion bath solution to inhibit post-OGD VRAC. All the fluorescence intensity values are arbitrary. **. The difference between the OGD and NPPB or DCPIB groups is statistically significant at <i>p</i>≤0.01t. <b>†</b>. The difference between the NPPB and DCPIB groups is statistically significant at <i>p</i>≤0.05.</p