OGD induced VRAC activation requires Na- +loading through glutamate receptors.

Abstract

<p><b>A</b>. In the presence of 40 µM AP-5 and 30 µM NBQX in the OGD solution to inhibit ionotropic glutamate receptors, the 25 min OGD induced neuronal electrophysiological changes seen in the <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0016803#pone-0016803-g003" target="_blank">Fig. 3A</a> were largely attenuated. Also, the activation of VRAC in the reperfusion stage was significantly inhibited. <b>B</b>. Shows the differences in the outward current amplitudes between 6 min and 20 min post-OGD under the following conditions: 1) control; 2) in the presence of DNDS+ bicuculline +furosemide in the OGD and 3) in the presence of AP-5+NBQX in the OGD solution. * Indicates that the difference between the control and AP-5+NBQX groups was statistically significant at <i>p</i><0.05.</p