Increased MMP-9 activity in culture supernatants from human PMNs stimulated with Ac-PGP.

Abstract

<p>Human PMNs isolated from peripheral blood were stimulated with Ac-PGP and IL-8 for different times and then supernatants were collected for MMP-9 assay. <b>A</b>. The detection of gelatinolytic activity in a time-dependent manner by g<i>elatin zymography</i> representative of three gels. <b>B</b>. The quantification of specific MMP-9 activity in a time-dependent manner by ELISA-based assay. <b>C</b>. The detection of gelatinolytic activity in a dose-dependent manner by g<i>elatin zymography</i> representative of three gels. <b>D</b>. The quantification of MMP-9 activity in a dose-dependent manner by ELISA-based assay. <i>*p<0.05</i>, compared with PMN only within same time point.</p