Phenotypic assays on wild type and <i>asb11^cul</i> embryos.

Abstract

<p>(<b>A</b>), Morphological analysis of wild type and mutant embryos at 48 and 72hpf. (<b>B</b>), (<i>left</i>) Anterior view of wild type and mutant embryos at 10hpf after whole mount <i>in situ</i> hybridization, WISH, using probe against <i>d-asb11</i>. (<i>right</i>) Graph shows the quantification of the respective expressions using qPCR. (<b>C</b>), (<i>left</i>) Endogenous d-Asb11 in wild type (WT), heterozygous (asb11+/−) and mutant (<i>asb11^cul</i>) embryos at 12 hpf was detected by immunoblotting using anti-d-Asb11 antibody. (<i>right</i>) Graph quantifies 3 individual experiments, with 30 embryos/genotype/experiment.</p