The effects of histamine and HR antagonists on cell viability in primary astrocytes. (A) The astrocytes were exposed to different concentrations of histamine (0.001–1 μg/ml) for 24 h. (B) The astrocytes were exposed to the H1R antagonist cetirizine (10 μM), the H2R antagonist ranitidine (10 μM), and the H3R antagonist carcinine (10 μM) and/or histamine (0.1 μg/ml) for 24 h. Cell viability was determined using a colorimetric method. Each data point represents the mean ± s.e.m. of at least three separate experiments in which treatments were performed in quadruplicates. (TIFF 507 kb
