Comparative Proteomic Analysis of Exosomes and Microvesicles
in Human Saliva for Lung Cancer
- Publication date
- Publisher
Abstract
Extracellular vesicles
(EVs) are cell-derived microparticles present
in most body fluids, mainly including microvesicles and exosomes.
EV-harbored proteins have emerged as novel biomarkers for the diagnosis
and prediction of different cancers. We successfully isolated microvesicles
and exosomes from human saliva, which were further characterized comprehensively.
Salivary EV protein profiling in normal subjects and lung cancer patients
was systematically compared through utilizing LC–MS/MS-based
label-free quantification. 785 and 910 proteins were identified from
salivary exosomes and microvesicles, respectively. According to statistical
analysis, 150 and 243 proteins were revealed as dysregulated candidates
in exosomes and microvesicles for lung cancer. Among them, 25 and
40 proteins originally from distal organ cells were found in the salivary
exosomes and microvesicles of lung cancer patients. In particular,
5 out of 25 and 9 out of 40 are lung-related proteins. Six potential
candidates were selected for verification by Western blot, and four
of them, namely, BPIFA1, CRNN, MUC5B, and IQGAP, were confirmed either
in salivary microvesicles or in exosomes. Our data collectively demonstrate
that salivary EVs harbor informative proteins that might be used for
the detection of lung cancer through a noninvasive way