Jasmonate Induced protein 60 (JIP60) was amplified from barley leaves infected with Blumeria graminis (Pennington et al., 2018). A peptide N-terminal domain was excised, and replaced with either a methionine-leucine (ML) linker, or a methionine, leucine, aspartic acid & proline linker (MLDP) to produce active forms of the JIP60 protein, Figure 1. Mak et al., (2007) had previously shown that these modifications were required to produce an active maize ribosome-inactivating protein (b-32). The Gateway entry construct pDONR_JIP60mldp is available from Addgene https://www.addgene.org/104959
