Additional file 1: of MicroRNA-1468 promotes tumor progression by activating PPAR-γ-mediated AKT signaling in human hepatocellular carcinoma

Abstract

Figure S1. Modulation of CITED2 or UPF1 mimics miR-1468-induced cellular processes in HCC. (A) Hep3B-miR-control cells that were transfected with empty vector (EV) or CITED2, UPF1 overexpression plasmid were subjected to western blot for CITED2 and UPF1. MHCC-97 L-anti-miR-NC cells that were transfected with scrambled siRNA or CITED2, UPF1 siRNA were subjected to western blot for CITED2, UPF1. CITED2 or UPF1 restoration mimics the effects of miR-1468 knockdown on cell proliferation (B, C), colony formation (D), cell cycle progression (E) and apoptosis (F) of Hep3B cells. CITED2 or UPF1 knockdown mimics effects of miR-1468 overexpression in MHCC-97 L cells (B-F). (G) Alteration of CITED2 or UPF1 mimics the effects of miR-1468 on cell cycle and apoptosis associated factors. *P < 0.05. (TIFF 704 kb

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