Detection of total, viable and membrane-perturbed bacteria using temporin A or temporin B labeled with rhodamine isothiocyanate or fluorescein isothiocyanate respectively.

Abstract

<p>A- B-C: <i>S.aureus</i> A 170 plus TA-Rho 6 µg plus TB-Fluo 9 µg; D-E-F: <i>S.aureus</i> A170 plus fluorescein isothiocyanate o rhodamine isothiocyanate (control); G-H-I: <i>S.enterica</i> serovar Paratyphi B plus TA-Rho 15 µg plus TB-Fluo 20 µg; L-M-N: <i>S.enterica</i> serovar Paratyphi B plus fluorescein isothiocyanate o rhodamine isothiocyanate (control).</p