Active JNK2 downregulated β-catenin expression and inhibited its transcriptional activity in a dose-dependent manner.

Abstract

<p>(A) Activated JNK2 reduced β-catenin protein level in a dose-dependent manner. HEK293T cells were co-transfected with pcDNA3-HA-β-catenin along with different amounts of pcDNA3-Flag-MKK7-JNK2, as indicated. Forty-eight hours after transfection, cells were harvested for immunoblotting analysis to detect the alterations of HA-β-catenin and p-JNK. β-actin served as loading control. (B) Activated JNK2 inhibited β-catenin-mediated transcriptional activity of TCF in a dose-dependent manner. HEK293T cells were co-transfected with pcDNA3-HA-β-catenin, TOPFLASH, Renilla, along with different amounts of pcDNA3-Flag-MKK7-JNK2, as indicated. Forty-eight hours after transfection, cells were harvested for luciferase activity assay. Each bar represents the mean ± standard deviation (SD) for triplicated samples.</p