R2E2 induces widespread aggregation of RΔ2-5.

Abstract

<p>(A–C) Co-expression of R2E2m::RFP promotes RΔ2-5m::YFP aggregation. Confocal images of (A) RΔ2-5m::YFP control and (B) RΔ2-5m::YFP co-expressed with R2E2m::RFP in BWM cells. Boxed area indicate representative region used for FRAP analysis. (C) FRAP analysis of RΔ2-5m::YFP foci in a line co-expressing RΔ2-5m::YFP and R2E2m::RFP. (D–F) Muscle-expressed R2E2m::RFP promotes intestinal RΔ2-5i::GFP aggregation. (D) Confocal image of control animal expressing RΔ2-5i::GFP in intestinal cells. (E) Confocal image of animals expressing R2E2m::RFP in BWM cells and RΔ2-5i::GFP in the intestine. Boxed areas indicate representative region analyzed by FRAP. (F) FRAP analysis of RΔ2-5i::GFP alone (1) and in animals co-expressing R2E2m::RFP and RΔ2-5i::GFP (2). RFI = relative fluorescence intensity in [%]. Scale bars: 10 µm.</p