Isolated Prom1⁺ embryonic lateral ventricle and SVZ cells in culture give rise to neurospheres expressing neural cell markers but not vascular markers.

Abstract

<p>Z-stack confocal images of cultured neurospheres generated from Prom1⁺ cells of E12.5 forebrain of prominin-1^lacZ/+ mice in serum free media and in the presence of bFGF revealed co-immunostaining for ß-galactosidase (green) and both Sox2 (A) and Musashi (B) stem cell markers. Z-stack confocal images of secondary differentiated neurospheres induced by removing bFGF and plating on polyL-ornithine and fibronectin coated dishes from Prom1⁺ cells of E16.5–E17.5 forebrain of prominin-1^lacZ/+ mice. Prom1⁺ cells (ß-galactosidase (green) in culture give rise to 3 different neural lineages (C) Olig 2 oligodendrocytes, (D) GFAP astrocytes and (E) Tuj1 neuron-specific progenitor neuronal marker.</p