<p>A. Schematic diagram of IRF-7C-specific epitope. The open bar represents open-reading frames (ORF). The expression plasmids were made as shown. The IRF-7C has unique 13 amino-acid (aa), represented by solid bar, at the C-terminus because the splicing changes the original ORF. The peptide was synthesized according to the last 15-aa sequence in IRF-7C and was used for antibody production. IRF-7CD is an expression plasmid that lacks the C-terminal 13-aa of IRF-7C. Specific epitope for IRF-7C is shown. The drawing is not on scale. B. Examination of IRF-7C-specific antibody. 293T cells were transfected with various expression plasmids as shown on the top. The expression levels of IRF-7C and GAPDH proteins were determined by Western blotting. Left panel: IRF-7C-specific antibody was used. Right panel: full-length IRF-7 antibody was used. C. IRF-7C protein is highly expressed in cells with EBV type III latency. Cell lysates from the indicated cells lines were separated by 12% SDS-PAGE. The expression levels of IRF-7C and tubulin proteins were determined by Western blotting. The images in the same box indicate that they are derived from the same membrane. The identities of proteins are shown.</p
