Immunofluorescence analysis of HFF cells infected with recombinant viruses.

Abstract

<p>The co-localisation of viral protein and EYFP in HFF infected with the recombinant virus was detected by fluorescence microscopy at 40× magnification. HFF were infected with TB4-IE2-EYFP (<b>A</b>), TB4-UL83-EYFP (<b>B</b>) or TB4-UL32-EYFP (<b>C</b>) with MOI 1 and fixated at 6, 24, 48 and 72 hpi as indicated on the left side. Cells were stained with mouse monoclonal antibodies directed against IE1–2 (E13) (A), ppUL83 (B) or ppUL32 (XP-1) (C) and Alexa-Fluor555 conjugated secondary antibody. Single channel recordings of DNA (Dapi), EYFP and the viral protein portion (red staining with Alexa-Fluor555) are shown in the left three columns; merged images are in the rightmost column. The time after infection is indicated on the left side.</p