Analytical HPLC analysis of purified antisense oligonucleotide.

Abstract

<p>Sample preparation: 25 µL purified antisense oligonucleotide; Column: DNAPac PA-100 (4/250); Flow rate: 1 ml/min; Buffer A: 10 mM NaClO₄+1 mM Tris; Buffer B: 300 mM NaClO₄+1 mM Tris; Gradient: 10–70% B, 7.6 CV.</p