The two PrgI ligand binding sites identified using FAST-NMR.

Abstract

<p>The two PrgI ligand binding sites are highlighted on an electrostatic potential surface (blue positive charge, red negative charge) calculated with DelPhi <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007442#pone.0007442-Nicholls1" target="_blank">[79]</a> implemented in Chimera <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0007442#pone.0007442-Pettersen1" target="_blank">[80]</a>. The didecyldimethylammonium bromide binding site (A) is found in a region responsible for needle formation while the chelerythrine binding site (B) is found on the opposite face.</p