The t(9;22) fusion proteins and their expression in Ph+ cells.

Abstract

<p>(A) Modular organization of the translocation partners and the fusion proteins in t(9;22). A schematic representation of the fusion proteins encoded by derivative 9 (der9) and 22 (der22), the Philadelphia chromosome, as well as of their combination in m-BCR-positive Ph+ ALL and M-BCR-positive CML, respectively. CC - coiled coil oligomerization interface; Y177 - Tyrosine phosphorylation site at aa 177; S/T kinase - serine/threonine kinase domain; DH - dbl homology domain; PH - pleckstrin homology domain; GAP - Rac-GAP domain; STEV - PDZ-domain binding motif; SH2 and SH3 - Src homology domains 2 and 3; Y kinase - tyrosine kinase domain; AB - actin binding domain. (B) Expression of the reciprocal ABL/BCR fusion proteins in Ph+ leukemia cell lines. Negative control: Phoenix cells; Tom-1, SD-1 and SupB15 - cell lines derived from m-BCR-positive ALL patient samples; BV173 - cells derived from a sample obtained from a CML patient in lymphatic BC; K562 - cells derived from a sample obtained from a CML patient in myeloid BC. (C) Expression of the reciprocal ABL/BCR fusion proteins in samples from Ph+ leukemia patients. Negative controls - three samples from AML patients and one sample from a CML patient for p96<i><sup>ABL/BCR</sup></i> in Ph+ ALL samples; one sample from an AML patient for p40<i><sup>ABL/BCR</sup></i> in CML samples.</p

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