Progeny from Wildtype and <i>DAZL</i>-Deficient Recipient Rats Transplanted with GCS-EGFP Rat Spermatogonia.

Abstract

<p>Wildtype or DAZL-deficient (DAZL-def) recipient rats were transplanted with either 0.5 or 1.5×10⁵ EGFP⁺ cells/testis from rat spermatogonial line GCS9 at 12 days after busulfan treatment (i.e. 12 mg/kg i.p.) on postnatal day 24. At ∼75 days post-transplantation recipients were paired with 75–80 day old wildtype female rats. Spermatogonia line GCS9 was harvested from passages number 13 and 17, which corresponded to 158 and 204 days in culture, respectively, prior to their transplantation. Recipients R942-R949 were littermates born from a hemizygous, transgenic DAZL-deficient female and a wildtype Sprague Dawley male. No progeny were born from breeder pairs of un-transplanted, busulfan-treated DAZL-deficient males and wild-type females (n = 3 breeder pairs). Breeder pairs of untreated, wild-type male litter mates of DAZL-deficient rats and wild-type female rats from Harlan, Inc. produced 15.5±4.5 pups/litter (+/−SEM, n = 8 litters from 3 breeder pairs).</p>1<p>p = 0.0209 Average Group 1 versus Average Group 2; p = 0.0251 Average Group 2 versus Average Group 3.</p>2<p>p = 0.0031 Average Group 1 versus Average Group 2.</p>3<p>Percent <i>GCS-EGFP</i>⁺ F1 progeny.</p