<p>(A) Replication of subgenomic luciferase replicons in transfected Huh-7 cells. The replication deficient replicon D318N served as negative control. Values refer to luciferase activities determined 48 h post transfection of Huh-7 cells after normalization for transfection efficiency determined 4 h post transfection. Data are taken from reference <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1000475#ppat.1000475-Lohmann3" target="_blank">[17]</a>. (B) Huh-7 cells were transfected with the Con1/wt genome or Con1 genomes carrying REMs specified in the bottom of the graph. The amount of core protein released into the supernatant 24 h after transfection was determined by using core ELISA and is expressed relative to the quantity of core measured for Con1/wt. Mean values of between 2 and 11 independent repetitions (depending on the construct) including the standard deviation are given. (C) Impact of REMs in NS4B and NS3 on RNA replication as determined by intracellular accumulation of core protein (left panel). The kinetic of release of core protein into the supernatant of Huh-7 cells after transfection with the Con1/wt or the Con1/K1846T or the Con1/NS3+K1846T genome is shown in the middle panel. The right panel shows the percentage of intracellular core that is released into the culture supernatant of Huh-7 cells at various time points after transfection with each of the 3 genomes. A representative example of two independent repetitions is shown.</p