<p>A. Southern blotting analysis of DNA from the WT and the YHΔ<i>tpx.</i> which was digested with EcoRV and PvuI and hybridized with a probe synthesized to make the complemented construct. B. PCR amplification of DNA from the WT and the YHΔ<i>tpx.</i> 1. WT, 2. <i>tpx</i> mutant. The primers used were designed in the coding region of the <i>tpx</i> gene. 3. WT, 4. <i>tpx</i> mutant. The primers used were for the amplification of complemented construct. M, molecular weight marker (Invitrogen). The experiments were repeated twice, with identical results.</p
