RIPK2 CARD forms homodimers/-oligomers.

Abstract

<p><i>Left panel</i>, Yeast two-hybrid analysis revealed that RIPK2 CARD (residues 427–527) forms homodimers/-oligomers (column 1 on SD/-4). Lamin c (Lam c) was used as control (column 5). <i>Right panel</i>, GST pull down assay. Specific binding of ³⁵S-labeled RIPK2 CARD (residues 427–527) was observed to recombinant expressed GST-RIPK2 CARD (residues 427–527), whereas binding to GST-Lam c was not detected. SD/-2: SD/-Leu/-Trp, SD/-4: SD/-Ade/-His/-Leu/-Trp.</p