Interaction of distinct NOD2 mutants with RIPK2.

Abstract

<p><i>Left panel</i>, Yeast two-hybrid analysis. NOD2 CARD1+2 interacts with RIPK2 CARD (column 1). No interactions were observed for NOD2 variants harboring a disrupted acidic patch (transformation 2, 3, and 4). An unrelated mutation not affecting the interaction interface had no effect (column 5). <i>Right panel</i>, Autoradiography of an <i>in vitro</i> transcription/translation of NOD2 proteins. NOD2 constructs were expressed using similar amounts of DNA and lysates.</p