Cellular uptake of a fluorescein labeled Aβ40 (F-Aβ40) and Alexa Fluor® 633 labeled transferrin (AF633-Trf) in wild type mouse brain slices after 30 min incubation.

Abstract

<p>A–B: Uptake of F-Aβ40 by a subpopulation of (A) cortical neurons (20×) and; (B) hippocampal neurons (20×). C–I: Effect of temperature on the uptake of F-Aβ40 and AF633-Trf by the pyramidal neurons (63× and 3× optical zoom). C–F: Uptake of F-Aβ40 and AF633-Trf at 37°C (C) F-Aβ40 uptake; (D) AF633-Trf uptake; (E) Superimposition of images C and D; (F) Limited co-localization of F-Aβ40 and AF633-Trf, indicated by white masked areas, was found only around the edges of pyramidal neurons. G–I: Uptake of F-Aβ40 and AF633-Trf at 4°C (G) F-Aβ40 uptake; (H) Inhibition of AF633-Trf internalization; (I) Superimposition of images G and H.</p