<p>A, B, C) F-actin (10 µM) polymerized without Tpm1p was incubated with 0, 10, 20 µM Cof1, Cof1-5 and Cof1-22 respectively and with 10 µM Tpm1p was incubated with 0, 2.5, 5, 10 and 20 µM Cof1, Cof1-5 and Cof1-22 respectively. The supernatants and pellets after ultracentrifugation (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003641#s4" target="_blank">Materials and Methods</a>) were analyzed on an SDS-PAGE gel. D) The cofilin/actin molar ratios in the pellet from two independent co-sedimentation experiments using 10 µM actin and 10 µM cofilin. Ratios were determined by densitometry of Coomassie-stained SDS-gels shown in (A), (B) and (C).</p
