Effect of H<sub>2</sub>O<sub>2</sub> on swelling-activated taurine release from cultured astrocytes and cortical synaptosomes.
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Abstract
<p>(a) The effect of H<sub>2</sub>O<sub>2</sub> on swelling-activated [<sup>14</sup>C]taurine release from cultured astrocytes. Astrocytes were preloaded overnight with [<sup>14</sup>C]taurine and D-[<sup>3</sup>H]aspartate. 300 µM H<sub>2</sub>O<sub>2</sub> was added to the media 10 min before and during exposure to hypoosmotic medium. For clarity, only [<sup>14</sup>C]taurine release is shown. The data are the mean values ±SEM of three experiments. **p<0.01 vs. hypotonic control. (b) The effect of H<sub>2</sub>O<sub>2</sub> on swelling activated [<sup>3</sup>H]taurine release from rat cortical synaptosomes. Integral [<sup>3</sup>H]taurine release was measured for 5 minutes as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0003543#s2" target="_blank">Material and methods</a>. 300 µM H<sub>2</sub>O<sub>2</sub> was present in media 10 min before and during measurements of taurine release. Data are the mean values ±SEM of three independent experiments performed in quadruplicate. ***p<0.001 vs. basal release.</p