Subcloning of 2 and 3 inserts into an expression vector.

Abstract

<p>(A–C) Maps of expression cloning vector pX-lacZ (A), and of entry clones and the resulting constructs for 2 or 3 insert cloning experiments (B, C respectively). The positions of the restriction sites for the enzymes XmaI and BsrGI are shown as black arrows, and the expected fragment sizes indicated. (D) Restriction digest (XmaI and BsrGI) of 12 minipreps for the two insert cloning (pX-GFP-H, lane 1 to 12) and the three insert cloning (pX-S-GFP-H, lane 13 to 24), and one miniprep of the vector (pX-lacZ, lane V).</p