Abstract

<p>(A, D, G and I) Zoom in view of the spots dynamic changes at different time points. Panel A, D, and G show three differential changed proteins in A549 model. The differential protein shown in panel I was from rat liver hepatectomy model. PH-I stands for the images were got from Phosphor-imaging, while CBB means the CBB staining. (B and E) Shown the dynamic expression changes of the spots labeled in panel A and D, respectively. The x axis is time (t), and the y axis is the protein synthesizing speed (v<sub>expressed</sub>), and the area of each rectangle means the total amount of protein synthesized during each 15 minutes interval (s<sub>expressed</sub>). The midpoint of each rectangle's top edge was used to interpolate these five points with piecewise cubic Hermite polynomial, as shown in the diagram as the green line. (C and F) Shown the dynamic total amount changes of the spots labeled in A and D. The x axis also is time (t), and the y axis is the total protein metabolized speed (v<sub>existed</sub>), consequently the area of each rectangle means the total amount of protein (s<sub>existed</sub>) variation during each time interval. This green line indicates the function of the variation speed of the total amount of existed protein (s<sub>existed</sub>) with respect to time (t), during these six hours. (H) Presence %Volumes changes of the protein labeled in chart G. Ph-R denotes phosphorylation rate, <i>i.e.</i> the percentage of phosphorylated proteins accounted for the total sum in each time point. The value of PH-R represents mean±s.e.m. for 3 independent experiments. Error bars represent mean±s.e.m., n = 3. Chart B, C, E and F were made by the software MatLab, and chart H was made by SPSS13.</p

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