The reduction of IFN-γ production among TCR β⁺NK1.1⁺ cells isolated from the liver of B7-1/2 deficient mice.

Abstract

<p>Eight-week old WT (top) and B7-1/2- (bottom) deficient mice received intravenous injection of Con A (25mg/kg). The mice were sacrificed six hours later. After perfusion the total number of mononuclear cells from the liver and spleen were stained with TCRβ, NK1.1, anti-mouse IFN-γ or IL-4 mAbs, or fluorescence conjugated isotype control. A. The percentage of total NKT cells (NK1.1⁺TCR β⁺) and conventional T cells (NK1.1⁺TCR β⁺) in the spleen and liver from Non-treated (left) and ConA treated mice (right). B. The percentage of cytokine producing cells among the NK1.1⁺TCR β⁺ population. C. The percentage of cytokine producing cells among the NK1.1⁻TCR β⁺ population. The numbers represent the percentage (Mean±SD) of one representative profile from each group is shown. The experiment was repeated twice; 7–8 mice were included in each group. D. Cytokine secretion of purified CD4 T cells from WT and B7KO mice stimulated by KRN7000. The numbers represent the profile of purified CD4 T cells from 3 mice in each group. The experiment was repeated twice.</p