Sn-expressing cells capture HIV-1<sub>NL4-3</sub> and enhance infectivity.
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Abstract
<p>TZM-bl cell cultures were seeded with various concentrations of HIV-1<sub>NL4-3</sub> (800–8000 pg/ml). Monocytic cells, TSn or THP-1 cells, were added and cocultured for 48 h. The capacity of Sn to capture HIV-1<sub>NL4-3</sub> in the cell culture medium and <i>trans</i> infect TZM-bl cells was analyzed for TSn, THP-1 and cell-free virus. Luciferase expression in HIV-1<sub>NL4-3</sub>-infected TZM-bl cells was quantified as relative light units (RLU). Results were compiled from 3 separate experiments. Error bars represent SD.</p
