Kinetics of naive and memory CD4⁺ T cells in the same mouse.

Abstract

<p>Wildtype mice containing 1.3×10³ naïve SMARTA (Thy1.1) and 1.3×10³ memory SMARTA (Ly5a) cells were given LCMV, and the relative abundance of the two SMARTA cell populations was determined by flow cytometry at various times post infection (two mice per time point). A. After gating on CD4⁺ T cells, the host CD4⁺ T cells (H), and the naïve and memory SMARTA cells (N & M respectively) were distinguished by Thy1.1 and Ly5a staining. The numbers indicate the frequencies of naïve and memory SMARTA cells as a percentage of all CD4⁺ T cells. B. The average±SE of the percentage of each population among all CD4⁺ T cells is shown over time. C. The total number of memory or naïve SMARTA CD4⁺ T cells per spleen is shown (average±SE).</p